一、基本信息
郁慧丽，博士，副教授，硕士生导师
电子邮件：huiliyu@hubu.edu.cn
研究领域：微生物次级代谢产物的生物合成，细胞色素P450酶挖掘，酶工程及绿色生物制造
二、教育背景
2013.10-2018.10 德国马尔堡大学，药学系 药物生物与生物技术研究所，博士
2010.9-2013.6 山东大学，微生物技术国家重点实验室，硕士
2006.9-2010.6 山东大学，必赢集团网站，学士
三、工作经历
2021.10-至今 必赢唯一官方网站，必赢集团网站，副教授
2020.12-2021.10 必赢唯一官方网站，必赢集团网站，讲师
2018.11-2019.11 德国马尔堡大学，药物生物与生物技术研究所，研究助理
四、主持科研项目
(1) 细胞色素P450催化合成二酮哌嗪二聚体的底物特异性分子机制解析和理性改造 国家自然科学基金青年项目 2025.1-2027.12，30万元，在研，主持
(2) 催化高附加值化合物合成的P450酶元件表征 上海智峪生科科技有限公司 2023.12-2028.12，27万元，在研，主持
(3) 生物合成α-羟基脂肪酸工艺的开发 上海云洛生物技术有限公司 2023.3-2024.3，10万元，结题，主持
(4) 轻工业用关键氧化还原酶的分子设计与高效生产，国家重点研发计划子课题 2021.7-2024.6，60万，结题，主持
(5) Streptomyces sp. 70中新颖环二肽合酶相关P450酶挖掘、功能及催化机制研究，湖北省教育厅，2021.1-2023.12，2万元，结题，主持
(6) 人工改造P450生产香精香料，必赢唯一官方网站校基金，2020.12-2021.12，2万元，结题，主持
五、代表性论文（#共同第一作者 *通讯作者）
1. Meng Dai#, Xiaojuan Yu#, Xiaogang Peng#, Zhiyong Guo*, Huili Yu*, Aitao Li Modulating regioselectivity of CYP107J3-catalyzed isophorone hydroxylation by disrupting the hydrophobic balance of the substrate binding pocket, Green Synthesis and Catalysis, 2024, https://doi.org/10.1016/j.gresc.2024.05.007
2. Huili Yu#, Xiaodong Zhang#, Carlos G Acevedo-Rocha, Aitao Li*, Manfred T Reetz* Protein engineering using mutability landscapes: Controlling site-selectivity of P450-catalyzed steroid hydroxylation, Methods in enzymology, 2023, 693, 191-229.
3. 郁慧丽，李爱涛* 细胞色素P450酶在香精香料绿色生物合成中的应用[J]. 生物技术通报，2023, 39 (4), 24-37.
4. Ruiwen Hu#, Anjie Gong#, Langxing Liao#, Yan‐Xin Zheng#, Xin Liu, Peng-Wu, Fushuai Li, Huili Yu, Jing Zhao, Long‐Wu Ye*, Binju Wang*, Aitao Li* Biocatalytic aminohydroxylation of styrenes for efficient synthesis of enantiopure β-amino alcohols. Chinese Journal of Catalysis, 2023, 44, 171–178.
5. Huili Yu; Xiulan Xie; Shu-Ming Li*; Coupling of cyclo-l-Trp-l-Trp with Hypoxanthine Increases the Structure Diversity of Guanitrypmycins, Organic Letters, 2019, 21(22): 9104-9108.
6. Huili Yu; Shu-Ming Li*; Two Cytochrome P450 Enzymes from Streptomyces sp. NRRL S-1868 Catalyze Distinct Dimerization of Tryptophan-Containing Cyclodipeptides, Organic Letters, 2019, 21(17): 7094-7098.
7. Huili Yu; Xiulan Xie; Shu-Ming Li*; Coupling of Guanine with cyclo-l-Trp-l-Trp Mediated by a Cytochrome P450 Homologue from Streptomyces purpureus, Organic Letters, 2018, 20(16): 4921-4925.
8. Jing Liu#; Huili Yu#; Shu-Ming Li*; Expanding tryptophan-containing cyclodipeptide synthase spectrum by identification of nine members from Streptomyces strains, Applied Microbiology and Biotechnology, 2018, 102(10):4435-4444.
9. Huili Yu; Mike Liebhold; Xiulan Xie; Shu-Ming Li*; Tyrosine O-prenyltransferases TyrPT and SirD displaying similar behavior toward unnatural alkyl or benzyl diphosphate as their natural prenyl donor dimethylallyl diphosphate, Applied Microbiology and Biotechnology, 2015, 99(17): 7115-7124.
10. Huili Yu#; Haoran Li#; Dongfang Gao; Cuijuan Gao; Qingsheng Qi*. Secretory production of antimicrobial peptides in Escherichia coli using the catalytic domain of a cellulase as fusion partner, 2015, Journal of Biotechnology, 214, 77-82.
11. Dongfang Gao; Shengjun Wang; Haoran Li; Huili Yu; Qingsheng Qi*. Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli, 2015, Microbial Cell Factories, 14, 49.
12. Shengjun Wang; Chuanliang Ma; Huili Yu; Changgan Huang; Qingsheng Qi*. Development of a method for the efficient release of N-glycans from glycoproteins generating native deglycosylated proteins, 2012, Enzyme and Microbial Technology, 51, 139-142.
13. 王圣钧, 郁慧丽, 翟琳, 王倚, 梁泉峰, 祁庆生*. 人GLP -1- IgG Fc融合蛋白在毕赤酵母中的高效表达[J]. 生物技术, 2011, 21(4), 29-33.
六、荣誉及奖励
2021 湖北省“楚天学子”
